100P Study on the effect of ursolic acid on MMPs and antimetastatic activity in TNBC cells

The matrix metalloproteinases (MMPs) are a family of proteolytic enzymes that degrade multiple components the extracellular matrix. significant role MMPs in tumor invasion, neoangiogenesis and metastasis presented it as ideal pharmacological targets for cancer therapy. effects ursolic acid (UA) on metalloproteinase (MMP) gene expression, cell invasion migration were investigated MDA MB- 231 line. Cytotoxicity UA towards cells was evaluated by MTT assay. cultured 48 hrs with different concentrations (0, 5, 15 25μM) mRNA expressions MMP-1, MMP-3, MMP-7, MMP-9 MMP- 11 analyzed real time PCR. Cell wound healing assay done at 0, 6, 24, h periods evaluation. To asses MB-231 seeded collagen based matrigel chamber treated 1, 10 15μM UA, whereas untreated considered control. After48 invaded through quantified colorimetric method. inhibited proliferation MB -231 dose dependent manner manner. Our results showed significantly affect all except MMP-3 7. expression MMP 3 reduced 0.34, 0.11 0.68 fold response to 25μM after 48hrs incubation comparison control cells. Wound exhibited hindered motility these 7.27% area remained unfilled 48hrs. 89.75% hrs. In conclusion, MDA-MB-231 Ursolic also TNBC